RNA Preparation and Purification
Roche Diagnostics KAPA mRNA HyperPrep Kit, 24 libraries
KAPA RNA HyperPrep Kits include all reagents required for RNA fragmentation, cDNA synthesis, library preparation and amplification. KAPA Pure Beads are supplied in each kit for reaction cleanups, while KAPA Adapters are available separately. Kits also include upfront depletion modules for mRNA capture (KK8580, KK8581), rRNA depletion (KK8560, KK8561), and rRNA and globin depletion (KK8562, KK8563). *This item is non returnable.
New England Biolabs, Inc. NEBNext Globin & rRNA Depletion Kit (Human/Mouse/Rat) with Sample Purification Beads – 96 reactions
The NEBNext Globin & rRNA Depletion Kit (Human/Mouse/Rat) employs the NEBNext RNase H-based RNA depletion workflow to deplete adult, embryonic and fetal globin mRNA (HBA1/2, HBB, HBD, HBM, HBG1/2, HBE1, HBQ1 and HBZ), cytoplasmic rRNA (5S, 5.8S, 18S, 28S, ITS and ETS) and mitochondrial rRNA (12S and 16S). The kit is effective with human, mouse and rat total RNA preparations, both intact and degraded. The resulting depleted RNA is suitable for RNA-seq, random-primed cDNA synthesis, or other downstream RNA analysis. This kit can also be used following poly(A) mRNA enrichment (e.g., using the NEBNext poly(A) mRNA Magnetic Isolation Module NEB #E7490), so that the final depleted RNA contains only mRNA of interest and no non-coding RNA. For convenience, this kit includes RNA Clean beads.
QIAGEN INC QIAAMP MINELUTE CCFDNA MINI KT
NC2470691 QIAAMP MINELUTE CCFDNA MINI KT
Molecular Research Center TRI REAGENT LS 200 ML
For single-step isolation of total RNA or simultaneous isolation of RNA, DNA and proteins from liquid samples. 0.75 ml of TRI Reagent LS processes 0.25 ml of liquid samples.
MyBioSource RNA (For fixed/embedded tissues)(without Phenol & Chloroform, need not DNase I digestion, directly use in RT-PCR or qPCR) Isolation Kit, 50 Tests
This kit is designed to quickly extract total RNA from formalin-fixed and paraffin-embedded tissue samples. The unique lysis solution/proteinase K quickly lyses cells to release RNA, and then the lysis mixture passes through a genomic DNA removal column, where genomic DNA is removed and RNA is filtered through. After the filtered RNA is adjusted with ethanol to adjust the binding conditions, the RNA is selectively adsorbed on the silicon matrix membrane in the spin column in a high-isolated salt state, and then through a series of rapid rinsing-centrifugation steps, the protein-removing solution and the rinsing solution remove the cells. Metabolites, proteins and other impurities are removed, and finally the low-salt RNase free H20 will elute the pure RNA from the silicon matrix membrane. The obtained RNA can be used in experiments such as reverse transcription PCR and fluorescent quantitative PCR.
Biochain Institute Inc Total RNA - Monkey (Rhesus) Normal Tissue: Small Intestine: Ileum, 50 ug/PK
BioChain's Total RNAs are isolated from a wide variety of documented human normal, diseased, and tumor tissues, mouse, rat, monkey, and plant tissues. Total RNA isolation is performed using proprietary techniques. Contamination by RNase, genomic DNA polysaccharides, and proteoglycans has been effectively eliminated. The integrity of the total RNAs is assured by checking for a ratio of greater than 1:1 between 28s and 18s ribosomal RNA. High efficiency reverse transcription using the Total RNA is demonstrated.
GINKGO BIOWORKS INC (3P) Ginkgo Bioworks™ Cell Free Protein Synthesis Premium kit (E. coli CFPS Premium): 100 mL
Ginkgo's CFPS Premium kit is Ginkgo's premier cell free protein synthesis (CFPS) product, optimized for the highest yields and most challenging proteins. This E. coli transcription/translation system is optimized to produce proteins from a T7 RNA Polymerase promoter. Reactions consist of a bacterial cell lysate and a reagent mix that contain all components required for protein production; the user simply provides a DNA template encoding the protein of interest under control of a T7 promoter. The kit also contains a Solubility Enhancer that can help improve expression and folding of challenging protein targets. Contents: E. coli lysate, Reagent Mix (3.3x), Solubility Enhancer (10x), and control plasmid. The 100 mL kit enables 5,000 reactions of 20 µL, or reaction volumes can be scaled to your specifications. Storage at -80° C. For research use only.
Biochain Institute Inc Total RNA - Rat Normal Tissue: Small Intestine, 50 ug/PK
BioChain's Total RNAs are isolated from a wide variety of documented human normal, diseased, and tumor tissues, mouse, rat, monkey, and plant tissues. Total RNA isolation is performed using proprietary techniques. Contamination by RNase, genomic DNA polysaccharides, and proteoglycans has been effectively eliminated. The integrity of the total RNAs is assured by checking for a ratio of greater than 1:1 between 28s and 18s ribosomal RNA. High efficiency reverse transcription using the Total RNA is demonstrated.
